Untitled Document

Question Bank No: 1

1. Which one of the following pairs of terms/names mean one and the same thing

a)		Gene pool- genome
b)		Codon - gene
c)		Cistron-triplet
d)		DNA finger printing- DNA profiling

2. Introduction of foreign gene for improving gene type is

a)		Tissue culture
b)		Genetic engineering
c)		Biotechnology
d)		Vernalisation

3. Construction of recombinant DNA involves

a)		Cleaving and rejoining of DNA segments with endonuclease
b)		Cleaving DNA segments with endonuclease and rejoining with ligase
c)		Cleaving and rejoining DNA segments with ligase
d)		Cleaving DNA segments with ligase and rejoining with endonuclease

4. Nucleic acid is fragmented by enzyme

a)		Ligases
b)		Proteases
c)		Nucleases
d)		Polymerases

5. An abnormal gene is replaced by normal gene. It is called

a)		Gene therapy
b)		Cloning
c)		Mutation
d)		None of the above

6. The enzyme capable of cutting DNA molecule at specific sites is

a)		Nuclease
b)		Restriction endonuclease
c)		Lipase
d)		Ligase

7. Restriction enzyme EcoR I cleavages DNA at the sequence

a)		AAGCTT
b)		AAGTTC
c)		GTATATC
d)		GAATTC

8. Which is related to genetic engineering?

a)		Plastid
b)		Plasmid
c)		Heterosis
d)		Mutation

9. Plasmids are used in genetic engineering because they are

a)		easily availabe
b)		able to replicate
c)		able to integrate with host chromosome
d)		inert

10. Restriction endonucleases are

a)		used in genetic engineering for uniting two DNA molecules
b)		used for in vitro DNA synthesis
c)		present in mammalian cells for degeneration of DNA of dead cells
d)		synthesised by bacteria for their defence

11. Two bacteria most useful in genetic engineering are

a)		Rhizobium and Azotobacter
b)		Escherichia and Agrobacterium
c)		Rhizobium and Diplococcus
d)		Nitrosomonas and Klebsiella

12. It is now possible to breed plants and animals of desired characters through

a)		Tissue culture
b)		Genetic engineering
c)		Ikebana technique
d)		Chromosome engineering

13. Restriction enzymes are used in genetic engineering because they

a)		can join DNA fragments
b)		cut DNA at specific base sequence
c)		cut DNA at variable sites
d)		Are proteolytic enzymes which degrade harmful proteins

14. Which one is correctly matched with its specificity for an amino acid, start or stop in protein synthesis

a)		UCG-start
b)		UUU-stop
c)		UGU-Leucine
d)		UAC-Tyrosine

15. Okazaki fragments are

a)		RNA primers
b)		short DNA fragments on leading strand
c)		short DNA fragments on lagging strand
d)		DNA fragments from dimerisation

16. Formation of mRNA over DNA template is

a)		Translation
b)		Transcription
c)		Reverse transcription
d)		Transduction

17. Wild type Escherichia coli growing on medium having glucose is transferred to lactose containing medium, which change occurs?

a)		Lac operon is induced
b)		Lac operon is suppressed
c)		All operons are induced
d)		The bacterium tops dividing

18. DNA tempalte sequence of CTGATAGC is transcribed over mRNA as

a)		GUCTUTCG
b)		GACUAUCG
c)		GAUTATUG
d)		UACTATCU

19. Transcription involves synthesis of

a)		DNA
b)		tRNA
c)		mRNA
d)		rRNA

20. A DNA with unequal nitrogen bases would most probably be

a)		Single stranded
b)		Double stranded
c)		Triple stranded
d)		Four stranded

21. Nuclcotide arrangement in DNA can be seen by

a)		X-ray crystallography
b)		Electron microscope
c)		Ultracentrifuge
d)		Light microscope

22. Experimental material in the study of DNA replication has been

a)		Escherichia coli
b)		Neurospora crassa
c)		Pneumococcus
d)		Drosophila melanogaster

23. DNA replication is

a)		Conservative and discontinuous
b)		Semiconservative and semidiscontinuous
c)		Semiconservative and discontinuous
d)		Conservative

24. One gene one enzyme hypothesis was proposed by

a)		Jacob and Monod
b)		Beadle and Tatum
c)		Watson and Crick
d)		Garrod and Jensen

25. Klinefelter's syndrome has number of chromosomes

a)		47
b)		46
c)		45
d)		44

26. Male is haploid in

a)		Lizard
b)		Cockroach
c)		Honey bee
d)		Bats

27. An inborn error of metabolism which eventually affects mental development is

a)		Albinism
b)		Phenylketonuria
c)		Anaemia
d)		Bleeder's disease

28. Number of barr bodies in XXXX female would be

a)		4
b)		3
c)		2
d)		1

29. According to genetic balance theory, X/A=1.5 will make the individual

a)		Male
b)		Metafemale
c)		Intersex
d)		None of the above

30. In humans, Philadelphia chromosome formed by reciprocal translocation between chromosomes

a)		9 and 21
b)		9 and 22
c)		9 and 20
d)		20 and 10

31. Genes exclusively present on Y-chromosome are called

a)		sex-linked
b)		Holandric
c)		Hologynic
d)		Histone

32. A woman has a child with Klinefelter's syndrome. Number of barr bodies present in the child is

a)		one
b)		Two
c)		three
d)		None

33. In Drosophila, XXY is female. In humans it represents an abnormal male because

a)		Y-Chromosome induces male traits in humans
b)		Y-chromosome is essential for female sex in Drosophila
c)		Y-chromosome is not essential for male sex in humans
d)		none of these

34. Determination of percentage of crossing over between two linked genes is important in

a)		maintaining heterozygosity in population
b)		indicating relative position of genes in chromosomes
c)		fixation of heterosis in organisms
d)		explaining the phenomenon of coupling and repulsion

35. Exchange of one part of a chromosome with a part of the same or another chromosome is

a)		Inversion
b)		crossing over
c)		Translocation
d)		Nucleolus

36. Euploidy is

a)		one chromosome more than haploid set
b)		one chromosome more than diploid set
c)		one chromosome less than haploid set
d)		Excat multiple of haploid set of chromosomes

37. A colour blind daughter is born in case of

a)		colour blind mother, normal father
b)		carrier mother, colour blind father
c)		Normal mother, colour blind father
d)		carrier mother, normal father

38. If BB represents barr body and $Y_{0} Y -$ body, XXY or Klinefelters syndrome has

a)		BB-1, $Y_{0} - 0$
b)		BB-1, $Y_{0}$ -1
c)		BB-0, $Y_{0}$ -1
d)		BB-2, $Y_{0}$ -1

39. Which one is de novo mutation?

a)		TT $\times TT \to TT$
b)		tt $\times tt \to Tt$
c)		Tt $\times tt \to Tt$
d)		Tt $\times Tt \to TT, Tt, tt$

40. Triticum aestivum (bread wheat) is

a)		Tetraploid
b)		Hexaploid
c)		Diploid
d)		Haploid

41. Smallest segment of genetic material affected by mutation is

a)		Recon
b)		Cistron
c)		Muton
d)		Exon

42. Which crop variety is not due to induced mutations?

a)		Reimei of rice
b)		Prabhat of Arhar
c)		Sharbati sonora of wheat
d)		Aruna of castor

43. A normal green male Maize is crossed with albino female. The progeny is albino is albino because

a)		Trait for albinism is dominant
b)		The albinos have biochemical to destroy plastids derived from green male
c)		Plastids are inherited from female parent
d)		Green plastids of male must have mutated

44. If haploid chromosome number is 10, the monosomic number shall be

a)		9
b)		18
c)		10
d)		19

45. Harmful mutations do not get eliminated from gene pool because

a)		They have survival value
b)		Genetic drift
c)		They are formed repeatedly
d)		They are recessive and carried by heterozygous individuals

46. Which one can induce polyploidy?

a)		Colchicine
b)		Acridines
c)		Ethylene
d)		Maleic hydrazide

47. Recessive mutations are expressed in

a)		Homozygous condition
b)		Heterozygous condition
c)		Next generation
d)		Same generation

48. Mutations used in agriculture are commonly

a)		Induced
b)		spontaneous
c)		Lethal
d)		recessive and lethal

49. Muller was awarded Nobel prize in 1946 for his work on

a)		Protein synthesis
b)		chemistry of nucleic acids
c)		cancer
d)		X-ray induced mutations

50. Trisomy has chromosome complement of

a)		2n-1
b)		2n-1-1
c)		2n+1+1
d)		2n+1